| Autor: |
Borgogna, JC, Shardell, MD, Santori, EK, Nelson, TM, Rath, JM, Glover, ED, Ravel, J, Gravitt, PE, Yeoman, CJ, Brotman, RM, Borgogna, J C, Shardell, M D, Santori, E K, Nelson, T M, Rath, J M, Glover, E D, Gravitt, P E, Yeoman, C J, Brotman, R M |
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| Zdroj: |
BJOG: An International Journal of Obstetrics & Gynaecology; Jan2020, Vol. 127 Issue 2, p182-192, 11p, 3 Graphs |
| Abstrakt: |
Objective: Characterise the vaginal metabolome of cervical HPV-infected and uninfected women.Design: Cross-sectional.Setting: The Center for Health Behavior Research at the University of Maryland School of Public Health.Sample: Thirty-nine participants, 13 categorised as HPV-negative and 26 as HPV-positive (any genotype; HPV+ ), 14 of whom were positive with at least one high-risk HPV strain (hrHPV).Method: Self-collected mid-vaginal swabs were profiled for bacterial composition by 16S rRNA gene amplicon sequencing, metabolites by both gas and liquid chromatography mass spectrometry, and 37 types of HPV DNA.Main Outcome Measures: Metabolite abundances.Results: Vaginal microbiota clustered into Community State Type (CST) I (Lactobacillus crispatus-dominated), CST III (Lactobacillus iners-dominated), and CST IV (low-Lactobacillus, 'molecular-BV'). HPV+ women had higher biogenic amine and phospholipid concentrations compared with HPV- women after adjustment for CST and cigarette smoking. Metabolomic profiles of HPV+ and HPV- women differed in strata of CST. In CST III, there were higher concentrations of biogenic amines and glycogen-related metabolites in HPV+ women than in HPV- women. In CST IV, there were lower concentrations of glutathione, glycogen, and phospholipid-related metabolites in HPV+ participants than in HPV- participants. Across all CSTs, women with hrHPV strains had lower concentrations of amino acids, lipids, and peptides compared with women who had only low-risk HPV (lrHPV).Conclusions: The vaginal metabolome of HPV+ women differed from HPV- women in terms of several metabolites, including biogenic amines, glutathione, and lipid-related metabolites. If the temporal relation between increased levels of reduced glutathione and oxidised glutathione and HPV incidence/persistence is confirmed in future studies, anti-oxidant therapies may be considered as a non-surgical HPV control intervention.Tweetable Abstract: Metabolomics study: Vaginal microenvironment of HPV+ women may be informative for non-surgical interventions. [ABSTRACT FROM AUTHOR] |
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Complementary Index |
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