| Autor: |
Chambo, Evelina, Ismae, Náalia, Vubil, Adolfo, Zicai, Ana Flora, Loquilha, Osvaldo, Mussá, Tufáaria, Mabunda, Nédio |
| Předmět: |
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| Zdroj: |
ISBT Science Series; Nov2019, Vol. 14 Issue 4, p395-400, 6p |
| Abstrakt: |
Background and Objectives The major interventions used to reduce the risk of HIV transmission from blood transfusion include the nucleic acid amplification assays (NAT). In Mozambique, screening for HIV infection in blood donors using serological assays is performed routinely. The aim of this study was to evaluate the use of a NAT test to screen blood donors at the blood bank of Hospital Central de Maputo. Material and Methods A cross-sectional study was conducted in a blood bank in the city of Maputo. Overall, 1500 blood donors were enrolled in the study from November 2014 to October 2015. The blood from each participant was drawn and used for both serological and HIV quantification. For HIV viral load quantification, mini-pools of six specimens were tested and, if positive, each sample was retested separately. Results Among the 1500 blood donors, the median age was 31 years old, 78·2% were men, and 74·7% were replacement donors. Our results showed a good sensitivity of (80/80) 100·0% (95·5-100%) and a specificity of (1408/1409) 99·9% (99·6- 100%) for the NAT when compared to the serological assay used at the blood bank. The kappa value of 0·99 (0·98-1·0) and confidence interval of 95·0% were observed indicating a good correlation between the NAT and serological assay. Conclusion Our data show that the HIV nucleic acid quantification assay has a good performance when compared to the serological assays, can detect infected donors/donations and can be used in mini-pools to minimize HIV infection resulting from the blood transfusion in the blood banks of Mozambique. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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