| Autor: |
ZENG Xin-hua, ZHAO Sheng-bo, LI Ke-qi, YUAN Rong, WU Gang, YAN Xiao-hong |
| Předmět: |
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| Zdroj: |
Chinese Journal of Oil Crop Sciences; 2019, Vol. 41 Issue 3, p340-348, 9p |
| Abstrakt: |
Allopolyploid Brassica napus contains a large number of homologous and redundant genes. It is difficulty to identify novel genes related to important agronomic traits by traditional T - DNA insertional, physical and chemical mutagenesis methods. In this study, a genome -wide inflorescence lhRNAi library of B. napus was constructed by the rolling circle amplification (RCA) -mediated lhRNAi construction technology. Subsequently, the inflorescence lhRNAi library was transformed into B. napus to produce 763 To transgenic plants. A total of 74 trans-genic lines exhibited visible mutant phenotypes, including petal reduction in the number, stamen degeneration shrinkage,stigma distortion and exposure, dead or closed buds and male sterility. These results demonstrated the construction method of lhRNAi library which were successfully applied to rapeseed and provided an important research platform for gene functions related to rape flower inflorescence development. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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