| Autor: |
Buhr, T.L., Minter, Z.A., Kennihan, N.L., Young, A.A., Borgers‐Klonkowski, E.L., Osborn, E.B., Bohmke, M.D., Hamilton, S.M., Kimani, M.B., Miller, C.T., Mackie, R.S., Innocenti, J.M., Bensman, M.D., Lilly, S.D. |
| Předmět: |
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| Zdroj: |
Journal of Applied Microbiology; Jan2020, Vol. 128 Issue 1, p124-137, 14p, 3 Diagrams, 2 Charts, 5 Graphs |
| Abstrakt: |
Aims: To add a spore germination step in order to reduce decontamination temperature and time requirements compared to the current hot, humid air decontamination parameters, which are 75–80°C, ≥72 h, 70–90% RH, down to ≤60°C and ≤24 h total decontamination time. Methods and Results: Bacillus anthracis spore germination with l‐alanine+inosine+calcium dipicolinate (CaDPA) was quantified at 0–40°C, several time points and spore concentrations of 5–9 log10 per ml. Germination efficiency at 0–40°C was >99% at <8 log10 spores per ml. The temperature optimum was 20°C. Germination efficiency was significantly higher but slower at 0°C compared to ≥30°C at ≥8 log10 spores per ml. A single germinant application followed by 60°C, 1‐h treatment consistently inactivated >2 log10 (>99%) of spores. However, a repeat application of germinant was needed to achieve the objective of ≥6 log10 spore inactivation out of a 7 log10 challenge (≥99·9999%) for ≤24 h total decontamination time for nylon and aircraft performance coating. Conclusions: l‐alanine+inosine+CaDPA stimulated germination across wide temperature and spore concentration ranges. Significance and Impact of the Study: Germination expands the scope of spore decontamination to include materials from any industry sector that can be sprayed with an aqueous germinant solution. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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