| Autor: |
Bilkhu, Satpal, El-Kayssi, Nazir, Poff, Matthew, Bushara, Anthony, Oh, Michael, Giustizia, Joseph, Kandalaft, Iyad, Lowe, Christine, Korol, Oksana, Sachs, Joel, Newton, Keith Glen, Macklin, James |
| Předmět: |
|
| Zdroj: |
Biodiversity Information Science & Standards; 2017, p1-2, 2p |
| Abstrakt: |
Agriculture and Agri-Food Canada (AAFC) is home to a world-class taxonomy program based on Canada's national agricultural collections for Botany, Mycology and Entomology. These collections contain valuable resources, such as type specimen for authoritative identification using approaches that include phenotyping, DNA barcoding, and whole genome sequencing. These authoritative references allow for accurate identification of the taxonomic biodiversity found in environmental samples in fields such as metagenomics. AAFC's internally developed web application, termed SeqDB, tracks the complete workflow and provenance chain from source specimen information through DNA extractions, PCR reactions, and sequencing leading to binary DNA sequence files. In the context of Next Generation Sequencing (NGS) of environmental samples, SeqDB tracks sampling metadata, DNA extractions, and library preparation workflow leading to demultiplexed sequence files. SeqDB implements the Taxonomic Databases Working Group (TDWG) Darwin Core standard Wieczorek et al. 2012 for Biodiversity Occurrence Data, as well as the Genome Standards Consortium (GSC) Minimum Information about any (X) Sequences (MIxS) specification Yilmaz et al. 2011. When coupled with the built-in data standards validation system, this has led to the ability to search consistent metadata across multiple studies. Furthermore, the application enables tracking the physical storage of the [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
