| Abstrakt: |
Many research showed the negative effects of diazinon (DZN) by inducing hepatorenal dysfunction. Therefore, this study was aimed to investigate the prophylactic and protective effects of Nigella sativa oil (NSO) against diazinon induced physiological and biochemical alterations in white male rats by biochemical tests (aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), total bilirubin (TOT.BIL), and total protein). 45 white male albino rats weighing (220–240 g) were used, rats were arbitrarily divided into five groups, (9 rats for every group). Group1 rats fed on traditional diet without treated as a control(CON). Rats in group 2 were treated with DZN via gavage in a dose of (10 mg/kg/daily) received was started at 15 days to the stop relating to the experiment. Rats in groups 3 were treated with NSO via gavage in a dose of (2 mg/kg/daily) received was started at 15 days to the stop relating to the experiment. Rats in group 4 were treated with DZN plus NSO via gavage and received was started at 15 days to the stop relating to the experiment. Rats in group 5 (prophylactic group) were treated with 2 ml/kg/every other day via gavage NSO in start from the first day for 14 days of the experiment and then DZN received at the above mentioned dose at 15 days to the end of the experiment. The consequences revealed to that a significant increased (P≤0.05) in AST, ALT, ALP, TOT.BIL activity of male rats treated with DZN compared with other groups and such an increase refers to hepatic dysfunction. And a significant increased (P≤0.05) in TP of CON group, (NSO)-treated group, (DZN+NSO)-treated group, and (NSO+DZN)-treated group, compared with (DZN)-treated group. Also, there were a significant increased (P≤0.05) in creatinine and urea in(DZN) treated group compared with the other groups, which refers to kidney injury. Our Study concluded that N. sativa oil pretreatment significantly reduced the diazinon induced hepatotoxicity, and nephrotoxicity. [ABSTRACT FROM AUTHOR] |
