| Autor: |
Ra, Sung-Ryong, Kim, Myong-Suk, Paek, Chon-IL, Pak, Yong-Chol, Pak, Song-Hui, Pak, Hyong-Bom, Ri, Kum-Chol |
| Zdroj: |
Folia Microbiologica; Nov2019, Vol. 64 Issue 6, p803-808, 6p |
| Abstrakt: |
Bacillus circulans 528 produces a restriction endonuclease, Bci528I which is an isoschizomer of EcoRI. We purified the enzyme, using Sephadex G-150, Phospho-cellulose, DEAE-cellulose, Hepharin-Sepharose CL-6B chromatography. The specific activity of Bci528I was 29,400 U/mg·protein. Bci528I recognizes 5′-GAATTC-3′ in dsDNA and cleaves between G and A of the recognition sequence, producing a symmetric four base 5′overhang. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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