| Autor: |
Pérez-Vázquez, María, Campoy, Pedro J Sola, Ortega, Adriana, Bautista, Verónica, Monzón, Sara, Ruiz-Carrascoso, Guillermo, Mingorance, Jesus, González-Barberá, Eva M, Gimeno, Concepción, Aracil, Belén, Sáez, David, Lara, Noelia, Fernández, Sara, González-López, Juan José, Campos, José, Kingsley, Robert A, Dougan, Gordon, Oteo-Iglesias, Jesús, Group, Spanish NDM Study, Sola Campoy, Pedro J |
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| Zdroj: |
Journal of Antimicrobial Chemotherapy (JAC); Dec2019, Vol. 74 Issue 12, p3489-3496, 8p |
| Abstrakt: |
Objectives: NDM carbapenemases have spread worldwide. However, little information exists about the impact of NDM-producing Enterobacteriaceae in Spain. By WGS, we sought to elucidate the population structure of NDM-like-producing Klebsiella pneumoniae and Escherichia coli in Spain and to determine the plasmids harbouring blaNDM-like genes.Methods: High-resolution SNP typing, core-genome MLST and plasmid reconstruction (PlasmidID) were performed on 59 NDM-like-producing K. pneumoniae and 8 NDM-like-producing E. coli isolated over an 8 year period in Spain.Results: Five major epidemic clones of NDM-producing K. pneumoniae caused five important nationwide outbreaks: ST437/NDM-7, ST437/NDM-1, ST147/NDM-1, ST11/NDM-1 and ST101/NDM-1; in contrast, the spread of NDM-producing E. coli was polyclonal. Three blaNDM types were identified: blaNDM-1, 61.2%; blaNDM-7, 32.8%; and blaNDM-5, 6%. Five K. pneumoniae isolates co-produced other carbapenemases (three blaOXA-48 and two blaVIM-1). The average number of acquired resistance genes was higher in K. pneumoniae than in E. coli. The plasmids encoding blaNDM-like genes belonged to IncFII, IncFIB, IncX3, IncR, IncN and IncC types, of which IncF, IncR and IncC were associated with MDR. The genetic surroundings of blaNDM-like genes showed a highly variable region upstream of ISAba125.Conclusions: In recent years NDM-producing K. pneumoniae and E. coli have emerged in Spain; the spread of a few high-risk K. pneumoniae clones such as ST437/NDM-7, ST437/NDM-1, ST147/NDM-1, ST11/NDM-1 and ST101/NDM-1 have caused several interregional outbreaks. In contrast, the spread of NDM-producing E. coli has been polyclonal. Plasmid types IncFII, IncFIB, IncX3, IncR, IncN and IncC carried blaNDM, and the same IncX3 plasmid was detected in K. pneumoniae and E. coli. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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