| Autor: |
Samatha, V., Devi, V. Rama, Satheesh, K., Subramanyam, K. V., Vinoo, R. |
| Předmět: |
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| Zdroj: |
Animal Science Reporter; Jul-Sep2019, Vol. 12 Issue 3, p1-11, 12p |
| Abstrakt: |
Ovine pulmonary adenocarcinoma (OPA), a transmissible lung tumor in sheep, caused by jaagsiekte sheep retrovirus (JSRV) can inflict fatality as high as 50% in the infected flock following initial detection of the disease. The routine diagnosis of OPA is entirely dependent on clinical signs that appear only at the terminal stages of the disease, followed by confirmation upon macroscopic and microscopic pathological investigations that requires sacrifice of the infected sheep. Serological tests are not available due to lack of JSRV specific antibodies. Polymerase Chain Reaction (PCR) is a useful tool for molecular diagnosis of OPA in sheep. This study aims to appraise the identification of OPA based on gross and histopathological lesions followed by molecular diagnosis of exogenous JSRV (ex- JSRV) by U3-hn PCR. A total of 150 lung samples of sheep suspected for OPA were collected from different slaughter houses and field mortalities in Andhra Pradesh for this investigation. Of these, 22 samples (14.7%) were positive for OPA based on gross histopathological findings and specific U3-hn PCR. Grossly, the affected lungs showed either consolidated areas or clearly demarcated tumor nodules in varying sizes. A few lungs revealed both. Histologically, papillary growth pattern was observed in 9 OPA lung samples. Acinar and bronchoalveolar growth patterns were observed in 7 and 4 lungs respectively. Two lungs showed more than one type of pattern in the same neoplastic tissue. Advanced lesions were characterized by dense fibrous tissue, thickened alveolar septa and infiltration of mononuclear cells in the interstitium. U3-hn PCR was conducted for molecular diagnosis and confirmation of OPA. An amplified product of 176bp was detected in positive samples. To improve the sensitivity, the product of the first round PCR was amplified by using a primer internal to the PCR product (P-VI) that was paired with primer P-I in the second round of hn PCR and a product of 133bp was detected. Electrophoretic analysis revealed the presence of bands of expected sizes in the PCR products in OPA positive animals. The study tends to conclude that U3-hn PCR may be used as a confirmatory inquest to detect the presence of ex-JSRV in OPA affected tissues in addition to gross and histopathological lesions and may be useful in epidemiological studies and disease control of OPA. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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