| Autor: |
de Souza, Felipe Augusto, Laguardia-Nascimento, Mateus, Gasparini, Marcela Ribeiro, Ferreira, Luciana Rabello, Sales, Érica Bravo, Cargnelutti, Juliana F., Camargos, Marcelo Fernandes, Fonseca Júnior, Antônio Augusto |
| Předmět: |
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| Zdroj: |
Archives of Virology; Dec2019, Vol. 164 Issue 12, p3095-3098, 4p |
| Abstrakt: |
Bovine alphaherpesvirus 2 (BoHV-2) is the etiologic agent of bovine mammillitis (BM) and pseudo-lumpy skin disease. BM is also important because its clinical presentation can be confused with foot-and-mouth disease (FMD), making it necessary to establish differential diagnoses and perform additional laboratory tests. The objective of this work was to use a validated real-time PCR assay to test for the presence of BoHV-2 in samples from cattle and buffalo with suspected vesicular disease in Brazil. The method could detect the virus at a concentration of 0.5 fg/μL and had 99.4% amplification efficiency, a repeatability error of only 4.1%, and good reproducibility with other reagents. No evidence of BoHV-2 causing vesicular disease in cattle and buffalo was found in this work. This study was able to validate a new methodology for detection of BoHV-2 and evaluate its usefulness for investigating outbreaks of vesicular disease Brazil. The importance of BoHV-2 in cases involving other clinical signs should still be studied using the qPCR developed in this work. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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