| Autor: |
Hanawa-Suetsugu, Kyoko, Itoh, Yuzuru, Ab Fatah, Maisarah, Nishimura, Tamako, Takemura, Kazuhiro, Takeshita, Kohei, Kubota, Satoru, Miyazaki, Naoyuki, Wan Mohamad Noor, Wan Nurul Izzati, Inaba, Takehiko, Nguyen, Nhung Thi Hong, Hamada-Nakahara, Sayaka, Oono-Yakura, Kayoko, Tachikawa, Masashi, Iwasaki, Kenji, Kohda, Daisuke, Yamamoto, Masaki, Kitao, Akio, Shimada, Atsushi, Suetsugu, Shiro |
| Předmět: |
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| Zdroj: |
Nature Communications; 10/18/2019, Vol. 10 Issue 1, pN.PAG-N.PAG, 1p |
| Abstrakt: |
Phagocytosis is a cellular process for internalization of micron-sized large particles including pathogens. The Bin-Amphiphysin-Rvs167 (BAR) domain proteins, including the FCH-BAR (F-BAR) domain proteins, impose specific morphologies on lipid membranes. Most BAR domain proteins are thought to form membrane invaginations or protrusions by assembling into helical submicron-diameter filaments, such as on clathrin-coated pits, caveolae, and filopodia. However, the mechanism by which BAR domain proteins assemble into micron-scale phagocytic cups was unclear. Here, we show that the two-dimensional sheet-like assembly of Growth Arrest-Specific 7 (GAS7) plays a critical role in phagocytic cup formation in macrophages. GAS7 has the F-BAR domain that possesses unique hydrophilic loops for two-dimensional sheet formation on flat membranes. Super-resolution microscopy reveals the similar assemblies of GAS7 on phagocytic cups and liposomes. The mutations of the loops abolishes both the membrane localization of GAS7 and phagocytosis. Thus, the sheet-like assembly of GAS7 plays a significant role in phagocytosis. The Bin/Amphiphysin/Rvs167 (BAR) domain superfamily, which includes FCH-BAR (F-BAR) domain proteins are membrane-sculpting proteins. Here the authors combine a range of techniques and show that the F-BAR domain of growth-arrest specific protein 7 (GAS7) forms two-dimensional sheets on flat membranes and that these oligomeric assemblies of GAS7 are required for the formation of phagocytic cups in macrophages. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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