Autor: |
MANTZOUKAS, SPYRIDON, DENAXA, NIKOLETA - KLEIO, LAGOGIANNIS, IOANNIS |
Předmět: |
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Zdroj: |
Current Agriculture Research Journal; 2019, Vol. 7 Issue 1, p37-45, 9p |
Abstrakt: |
Susceptibility of Tuta absoluta Meyrick (Lepidoptera : Gelechiidae) populations to Metarhizium anisopliae and Bacillus thuringiensis (Bt) were evaluated under laboratory conditions. T. absoluta larvae were treated either individually or in combination with a single dose rate of B. thuringiensis (0.5 μL/L) and three conidial suspensions viz. 1 x 104, 1 x 106 and 1 x 108 spores / mL of M. anisopliae. Larval mortality, pupation, adult emergence, mycosis and sporulation varied depending on the application of different quantities of M. anisopliae alone and in combination with B. thuringiensis. Maximum mortality (100%) was achieved in 2nd instar larvae when M. anisopliae (1 x 108 spores/mL) and B. thuringiensis (0.5μL/L) were applied synergistically, while 4th instar larvae recorded a 95.45% mortality. Compared with the untreated checks, mortality, pupation and adult emergence of both 2nd and 4th instar larvae were significantly reduced with the combined application of M. anisopliae (1 x 108 spores / mL) and B. thuringiensis (0.5μL/L). Mycosis was most prevalent on 2nd and 4th instar larvae, exceeding 88 and 80% respectively, after exposure to 1 x 104 conidia / mL. The results indicate that the entomopathogenic fungi and the insecticidal protein produced by B. thuringiensis can be used in combination as biocontrol agents for the management of T. absoluta. [ABSTRACT FROM AUTHOR] |
Databáze: |
Complementary Index |
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