| Autor: |
Пілінська, М. А., Курінний, Д. А., Рушковський, С. Р., Дибська, О. Б. |
| Předmět: |
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| Zdroj: |
Problems of Radiation Medicine & Radiobiology; 2016, Issue 21, p141-148, 8p |
| Abstrakt: |
Objective: to identify possible radioprotective properties of astaxanthin by means of cytogenetic criteria. Methods. Cultivation of peripheral blood lymphocytes from five apparently healthy volunteers; treatment of lym& phocytes' cultures by astaxanthin in final concentrations 20 μg/ml in Go phase of mitotic cycle, prior to & irradia& tion in vitro in a dose 1 Gy; cytogenetic analysis the uniformly stained slides of metaphase chromosomes. The elec& trophoresis of individual cells (Comet assay); visualization of results under fluorescent microscope; accounting the number of nucleoid the fourth grade that correspond to apoptosis of the cells. Results. Established that astaxanthin in final concentration 20.0 μg/ml exposed to the culture of human peripher& al blood lymphocytes in the early G0 phase of mitotic cycle leads to significant reduction of cytogenetic effects induced by gamma irradiation in vitro in dose 1.0 Gy (from 26.05 ± 1.81 to 9.08 ± 0.78 per 100 cells, respectively) and to significant increase the frequency of apoptotic cells at the 48 hour of cultivation (from (3.78 ± 0.24) to (8.26 ± 0.91) %, respectively). Conclusions. The results obtained show the ability of astaxanthin to considerable weakening of radioinduced muta& genic effect in human peripheral blood lymphocytes, which testify its powerful radioprotective potential. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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