Bright split red fluorescent proteins for the visualization of endogenous proteins and synapses.

Autor: Feng, Siyu, Varshney, Aruna, Coto Villa, Doris, Modavi, Cyrus, Kohler, John, Farah, Fatima, Zhou, Shuqin, Ali, Nebat, Müller, Joachim D., Van Hoven, Miri K., Huang, Bo
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Zdroj: Communications Biology; 9/17/2019, Vol. 2 Issue 1, pN.PAG-N.PAG, 1p
Abstrakt: Self-associating split fluorescent proteins (FPs) are split FPs whose two fragments spontaneously associate to form a functional FP. They have been widely used for labeling proteins, scaffolding protein assembly and detecting cell-cell contacts. Recently developments have expanded the palette of self-associating split FPs beyond the original split GFP1-10/11. However, these new ones have suffered from suboptimal fluorescence signal after complementation. Here, by investigating the complementation process, we have demonstrated two approaches to improve split FPs: assistance through SpyTag/SpyCatcher interaction and directed evolution. The latter has yielded two split sfCherry3 variants with substantially enhanced overall brightness, facilitating the tagging of endogenous proteins by gene editing. Based on sfCherry3, we have further developed a new red-colored trans-synaptic marker called Neuroligin-1 sfCherry3 Linker Across Synaptic Partners (NLG-1 CLASP) for multiplexed visualization of neuronal synapses in living C. elegans, demonstrating its broad applications. Feng et al develop a system based on split fluorescent proteins with enhanced complementation efficiency, characterise further the complementation mechanism and illustrate its use by visualising endogenous synapsis in C.elegans though the tagging of endogenous proteins. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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