| Autor: |
Chen, Duo, Yuan, Xue, Liang, Limin, Liu, Kui, Ye, Haoying, Liu, Zhiyu, Liu, Yanfei, Huang, Luqiang, He, Wenjin, Chen, Youqiang, Zhang, Yanding, Xue, Ting |
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| Zdroj: |
Biotechnology Letters; Oct2019, Vol. 41 Issue 10, p1133-1145, 13p |
| Abstrakt: |
Chlamydomonas reinhardtii is a photosynthetic unicellular model algae with multiple biotechnological advantages, and its fatty acids can be used to produce biofuels. Numerous studies suggest that acetyl-coA carboxylase (ACCa) catalyzes the first committed and rate-limiting step of fatty acid biosynthesis, thereby playing a central role in oil accumulation. Here, we cloned and overexpressed ACCa in C. reinhardtii to directly evaluate its effect on fatty acid synthesis. GC–MS analysis found that the unsaturated FAs contents of the CW15-24 and CW15-85 strains were 55.45% and 56.15%, which were significantly enriched compared to the wild type CW15 (48.39%). Under the optimized conditions, the content of lipid by overexpressed the ACCa gene in the mutant CW15-85 (0.46 g/l) was 1.16-fold greater than control through optimization of N and P sources. Altogether, our data clearly demonstrate that ACCa overexpression in C. reinhardtii can directly increase the synthesis of fatty acids. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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