| Autor: |
Thingholm, Karen Rønø, Hertz, Frederik Boëtius, Løbner-Olesen, Anders, Frimodt-Møller, Niels, Nielsen, Karen Leth |
| Předmět: |
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| Zdroj: |
Infection & Drug Resistance; Aug2019, Vol. 12, p2429-2435, 7p |
| Abstrakt: |
Background: Extended spectrum beta-lactamase (ESBL)-producing Escherichia coli (E. coli) causing urinary tract infections often belong to sequence type 131 (ST131), serotype O25, carrying blaCTX-M-15. Aim: The main aim of this study was to examine the conjugational frequencies of E. coli with plasmids carrying blaCTX-M-15 to E. coli isolates from the fecal flora of healthy humans to determine whether ST131 is more likely to uptake or donate ESBL resistance compared to other E. coli clones. Methods: Donors and recipients were all clinical isolates and did not harbor plasmids with identical incompatibility groups (Inc-groups) based on in silico analyses of Inc-groups and restriction/modification systems (R/M-systems). The in vitro conjugation experiments were performed as filter conjugation with verification of transconjugants by random amplified polymorphic DNA (RAPD) PCR and blaCTX-M-15 PCR. Results: The frequencies of conjugation with blaCTX-M-15-carrying plasmids were found to be very rare with detectable conjugation frequencies in the range of 4x10−9–7x10−7 transconjugants/recipient. Recipients of O25/ST131 type yielded significantly lower conjugation frequencies compared to recipients of other O-types (P=0.004). The applied ST131/O25 donors did not yield detectable levels of transconjugants regardless of the applied recipient. Presence of sub-MIC levels of ampicillin increased plasmid transfer frequencies x100 fold (P=0.07). Conclusion: The results indicate that blaCTX-M-15 is rarely transferred by conjugation to E. coli isolates of the intestinal flora, even when the gene is plasmid-borne. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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