| Autor: |
Lorenzo, M. S., Maruri, A., Cruzans, P. R., Teplitz, G. M., Tello, M. F., Lombardo, D. M. |
| Předmět: |
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| Zdroj: |
Reproduction, Fertility & Development; 2019, Vol. 31 Issue 10, p1607-1615, 9p |
| Abstrakt: |
Abattoir ovaries, which are the main source of oocytes for reproductive biotechnologies, arrive at the laboratory under ischaemic conditions. Reoxygenation generates reactive oxygen species (ROS) in ischaemic tissues, which could affect oocyte quality. The aim of this study was to evaluate the effect of supplementation of media with dimethylthiourea (DMTU) during the collection and washing of cumulus–oocyte complexes (COC) on ROS levels, COC apoptosis and oocyte nuclear and cytoplasmic maturation. Thus, the collection (TCM-199) and washing (TCM-199 with 10% porcine follicular fluid, sodium pyruvate and antibiotics) media were supplemented with 1 and 10 mM DMTU. In the control group, the media were not supplemented with DMTU. Intracellular ROS levels decreased significantly in the DMTU-treated groups (P < 0.05). Although no effects on rate of nuclear maturation were observed, DMTU significantly increased sperm penetration rates without increasing polyspermy (P < 0.05). The addition of 10 mM DMTU to the collection and washing media enhanced IVF efficiency. DMTU did not modify the early or late apoptosis of oocytes. Both concentrations of DMTU significantly increased viability and decreased the apoptosis of cumulus cells (P < 0.05). These results suggest that the addition of 1 or 10 mM of DMTU to the media during the collection and washing of porcine COCs is useful for decreasing cumulus apoptosis mediated by ROS and for optimising the IVF of porcine oocytes. This is the first report of the addition of an antioxidant during porcine cumulus–oocyte complex collection and washing. The antioxidant dimethylthiourea decreased reactive oxygen species in porcine oocytes, improved IVF efficiency and reduced cumulus apoptosis. Redox balance is key for successful embryo production. Thus, dimethylthiourea may be a tool for improving oocyte quality and embryo development. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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