| Autor: |
Lindsay, James A., Sleigh, Robert W., Ghitgas, Christine, Davenport, James B. |
| Předmět: |
|
| Zdroj: |
European Journal of Biochemistry; 6/3/85, Vol. 149 Issue 2, p287-293, 7p |
| Abstrakt: |
A method is described for isolating an enterotoxin from a coatless spore tauten t (8–6) of Clostridium perfringens type A. The characteristics of this enterotoxin only slightly resembled those of previously isolated enterotoxins of C. perfringens. The type A (8–6) enterotoxin was found to be composed of two subunits of Mr 18000 with isoelectric points of 3.8 and 4.3. The LD50 for mice was 39 μg/kg with 0.10 μg corresponding to one erythemal unit. The type A (8–6) enterotoxin was inactivated by heating for 10 rain at 60°C. The amino acid composition data of type A (8-6) and delta toxins was similar, but type A (8-6) and type A enterotoxins showed less similarity. This lack of similarity between type A and type A (8–6) enterotoxins was confirmed by the failure of anti-sera to type A enterotoxin to neutralize the type A (8–6) enterotoxin, in both the mouse and erythemal tests. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
