| Autor: |
Nunez, Corina, Tondini, Sara M, Jennings, Thomas G, Rickard, Justin W, Green-Miller, Angela R, Shike, Daniel W, Henry, Darren D, McCann, Joshua C |
| Předmět: |
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| Zdroj: |
Journal of Animal Science; 2019 Supplement, Vol. 97, p240-241, 2p |
| Abstrakt: |
The objective was to evaluate treatments designed to increase passage rate on the in vitro methanogenic potential of rumen and fecal contents. Eight ruminal-cannulated steers were used in a replicated 4×4 Latin square design with data collected from the latter three 24 d periods. All steers were fed an ad libitum diet of grass hay (10.4% CP) with the following treatments designed to increase passage rate: negative control (CON), pelleted (PEL), 7% Ca(OH)2 treated (CAOH), and four 6 kg weights added to the rumen (WTS). Molasses (3% of diet DM) was added to all treatments to improve diet palatability. On d 22 of each period, fecal and rumen samples for in vitro procedure were collected 0 and 2 hr after feeding, respectively. Rumen (10 g) and fecal (20 g) samples were added to 100 ml Wheaton vials with buffer solution at 1:5 and 1:2 ratio, respectively. Vials were incubated at 39°C, and samples were collected at 6 and 24 hr to determine gas production. Methane was analyzed using gas chromatography. Data were analyzed using the MIXED procedure of SAS. In vitro methane production rate from rumen samples tended (P = 0.09) to be affected by treatment with the greatest rate observed for CON (11.7 mM per g DM per h) and the least for PEL (6.3 mM per g DM per h). Rate of fecal methane production in vitro was not affected (P = 0.48) by treatment with means ranging from 0.79 to 2.3 mM per g DM per h. Predicted daily methane production from fecal dry matter excretion was also not affected (P = 0.98) by treatment. Treatments designed to increase passage rate may affect the methanogenic potential of archaea in ruminal contents but not in feces. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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