| Autor: |
Aranha Berto, Luciana, Lauener, Anic, Saads Carvalho, Thiago, Lussi, Adrian, Eick, Sigrun, Berto, Luciana Aranha, Carvalho, Thiago Saads |
| Předmět: |
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| Zdroj: |
Oral Health & Preventive Dentistry; 2019, Vol. 17 Issue 4, p375-383, 9p |
| Abstrakt: |
Purpose: The effects of arginine as a toothpaste additive were assessed on oral streptococci with and without a known arginine deiminase system (ADS) and cariogenic biofilms.Materials and Methods: Suspensions of Streptococcus mutans, S. sobrinus and the ADS-positive (ADS+) S. sanguinis and S. gordonii were cultured with or without 1.5% L-arginine for 24 h. Thereafter, biofilms consisting of the four species were formed on polystyrene surfaces with or without 1.5% L-arginine for up to 10 d. Finally, biofilms that formed on enamel surfaces were exposed to a daily mechanical cleaning with an arginine and sodium monofluorophosphate (SMF+Arg)-containing toothpaste, a sodium monofluorophosphate fluoride (SMF)-containing toothpaste or a negative control for up to 10 weeks. At different incubation times, the pH in the culture media, the citrulline production and the percent of ADS+ bacteria within the biofilms were determined. Microsurface hardness loss was quantified in the experiments using enamel specimens.Results: In the presence of 1.5% arginine, S. sanguinis and S. gordonii showed a high level of production of citrulline after 6 h of incubation, together with an increase in the pH when compared to S. mutans and S. sobrinus. With arginine supplementation, the percentage of ADS+ species was higher at 1, 2 and 4 days and citrulline production was higher at all days of biofilm formation on polystyrene surfaces. After 4 and 10 weeks of treating biofilms on enamel surfaces, the SMF+Arg group had a higher proportion of ADS+ strains than the SMF group; at 4 weeks, the pH was higher in the SMF+Arg group. Loss of enamel hardness was the lowest in the SMF+Arg group and was significantly less in the SMF+Arg group than in the control group after 2, 4 and 10 weeks of treatment.Conclusion: Toothbrushing using an arginine-containing toothpaste may protect against dental caries. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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