| Autor: |
Shen, Lu, Chen, Yuan, Cheng, Jing, Yuan, Suzhen, Zhou, Su, Yan, Wei, Liu, Junfeng, Luo, Aiyue, Wang, Shixuan |
| Předmět: |
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| Zdroj: |
Journal of Cellular Physiology; Dec2019, Vol. 234 Issue 12, p22554-22564, 11p |
| Abstrakt: |
As a fundamental aging mechanism, cellular senescence causes chronic inflammation via the senescence‐associated secretory phenotype (SASP). Theca‐interstitial cells are an essential but little‐studied component of follicle development in the ovarian microenvironment. In the present study, we observed significant cellular senescence in theca‐interstitial cells and secretion of chemokine (C‐C motif) ligand 5 (CCL5) by these cells during aging. Furthermore, we aimed to investigate whether and how senescence‐associated secretory phenotype (SASP)‐associated CCL5 may be involved in follicle development. Increased levels of CCL5 in the microenvironment of follicles attenuated preantral follicle growth, survival, and estradiol secretion. Oocyte maturation and the expression of zona pellucida 3 and differentiation factor 9 (GDF9) were also inhibited by CCL5. Granulosa cell apoptosis in follicles was promoted by CCL5, accompanied by the phosphorylation of nuclear factor‐κB by CCL5 and inhibition of the PI3K/AKT pathway. These results suggest that SASP‐associated CCL5 produced by senescent theca‐interstitial cells may impair follicle development and maturation during ovarian aging by promoting granulosa cell apoptosis. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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