| Autor: |
Shaposhnikova, Galina I., Prokazova, Nina V., Buznikov, Gennadiy A., Zvezdina, Nataliya D., Teplitz, Ninel A., Bergelson, Lev D. |
| Předmět: |
|
| Zdroj: |
European Journal of Biochemistry; 5/2/84, Vol. 140 Issue 3, p567-570, 4p |
| Abstrakt: |
The ganglioside composition of mouse ascites hepatoma (MAH) cells, the ascites fluid and cell-conditional media were determined and found to be qualitatively identical, but quantitatively different. The ganglioside content of the ascites fluid and the medium conditioned by MAH-cells at the native cell concentration (1o8 cells/nm) comprised respectively 74.8% and 23% of the cell-associated gangliosides. When incubated at lower cell-density (106 cells/ml) the cells were found to be release about three-times higher amounts of ganglioside per cell than during incubation at the native concentration. Centrifugation of the dense-cell-conditioned medium revealed the major part of the released gangliosides to be associated with a 150 000 x g pellet that probably contains shed plasma membrane fragments. In the 150 000 x g pellet of the extracellular fluids the relative content of the most polar cell ganglioside corresponding chromatographically to GT1b was about ten-times higher than in the cells. The possibility is raised that the more intense shedding of gangliosides from less crowded MAH cells may play a role in the self protection of the tumor from host immune rejection during initial stages of growth. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
