| Autor: |
Langley, Keith E., Por-Hsiung Lai, Wypych, Jette, Everett, Richard R., Berg, Thor F., Krabill, L. F., Davis, Janice M., Souza, Lawrence M. |
| Předmět: |
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| Zdroj: |
European Journal of Biochemistry; 3/2/87, Vol. 163 Issue 2, p323-330, 8p, 2 Diagrams, 2 Charts, 7 Graphs |
| Abstrakt: |
Bacterially synthesized, recombinant-DNA-derived bovine growth hormone (r-bGH), prepared as described in the preceding paper in this journal, has been characterized in comparison with pituitary bovine growth hormone (pit-bGH). The characterization criteria include sodium dodecyl sulfate/polyacrylamide gel electrophoresis, automated N-terminal sequence analysis, amino acid composition, isoelectric focusing, reverse-phase high- performance liquid chromatography, ultraviolet absorbance, analysis for free protein thiol, sizing by gel filtration, circular dichroism, radioimmunoassay and biological activity in the hypophysectomized rat weight-gain assay. In every respect the r-bGH appears to be virtually identical to pit-bGH. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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