Expression and regulation of neuronal apoptosis inhibitory protein during adipocyte differentiation.
| Autor: | Magun, Rick, Gagnon, AnneMarie, Yaraghi, Zahra, Sorisky, Alexander, Magun, R, Gagnon, A, Yaraghi, Z, Sorisky, A |
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| Předmět: |
FAT cells
APOPTOSIS PROTEINS CHEMICAL inhibitors CELL metabolism PROTEIN metabolism ANIMAL experimentation ANTIBIOTICS CELL differentiation CELL receptors CELLS COMPARATIVE studies CULTURE media (Biology) GENETIC techniques HYPOGLYCEMIC agents INSULIN RESEARCH methodology MEDICAL cooperation MICE NERVE tissue proteins RATS RESEARCH TRANSCRIPTION factors RAPAMYCIN EVALUATION research PHARMACODYNAMICS |
| Zdroj: | Diabetes; Dec1998, Vol. 47 Issue 12, p1948-1952, 5p, 1 Black and White Photograph, 5 Diagrams |
| Abstrakt: | We have used the 3T3-L1 and 3T3-F442A preadipocyte cell lines to examine the expression and regulation of neuronal apoptosis inhibitory protein (NAIP) during adipocyte differentiation. When 3T3-L1 preadipocytes differentiated into adipocytes, they developed resistance to apoptosis induced by growth factor deprivation, as assessed by terminal deoxynucleotide transferase (TdT)-mediated dUTP nick end labeling. Protein expression of NAIP was markedly elevated in 3T3-L1 and 3T3-F442A adipocytes compared with that in their fibroblast-like precursors. NAIP was also present in rat white adipocytes. In 3T3-L1 cells, the increase in NAIP occurred by day 4 of the 8-day differentiation protocol, which includes exposure of confluent preadipocytes to insulin, dexamethasone, and isobutylmethylxanthine. Incubation of confluent 3T3-L1 preadipocytes with any of these components alone had no effect on NAIP expression. When 3T3-C2 cells, a control cell line that does not differentiate, were subjected to the differentiation protocol, the low NAIP levels remained unaltered. Addition of rapamycin, a p70 S6 kinase inhibitor that blocks adipocyte differentiation, to the 3T3-L1 differentiation medium prevented the rise in NAIP expression. These data demonstrate for the first time that NAIP is expressed in adipocyte cell lines and primary adipocytes. The differentiation-dependent augmentation of NAIP protein levels in 3T3-L1 adipocytes is closely correlated with the development of resistance to apoptosis induced by growth factor deprivation, suggesting a potential role for NAIP in these cells. [ABSTRACT FROM AUTHOR] |
| Databáze: | Complementary Index |
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