The potential effect of APTR amplification on glioma patients' prognosis.
| Autor: | ZHANG Shan-shan, YU Lin |
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| Předmět: |
ACADEMIC medical centers
CELL proliferation BIOLOGICAL assay CANCER patients CELL physiology CHI-squared test GENE amplification GENE expression GLIOMAS HETEROCYCLIC compounds MULTIVARIATE analysis POLYMERASE chain reaction PROTEINS RNA STATISTICS REVERSE transcriptase polymerase chain reaction DESCRIPTIVE statistics |
| Zdroj: | Chinese Journal of Contemporary Neurology & Neurosurgery; Jul2019, Vol. 19 Issue 7, p507-513, 7p |
| Abstrakt: | Objective To explore the potential effect of long non-coding RNA APTR gene amplification on glioma cell proliferation and invasion and glioma patients' prognosis. Methods Quantitative real - time polymerase chain reaction (qRT - PCR) was used to analyze the APTR gene amplification and expression condition of 30 cases of World Health Organization (WHO) grade II-IV glioma patients from Tianjin Medical University General Hospital, and the APTR amplification and expression in 1119 cases of glioma patients from The Cancer Genome Atlas (TCGA) database. The correlation of APTR amplification rate with glioma grade, APTR LncRNA level and patients' prognosis was accessed. The effects of APTR gene on proliferation and invasion in U251 glioma cells were further confirmed by methyl thiazolyl tetrazolium (MTT) assay and Transwell assay. Results The APTR amplification rate was positively correlated with glioma grade, the results were significant (χ² = 53.901, P = 0.000; χ² = 267.832, P = 0.000; χ² = 118.412, P = 0.000). APTR amplification rate was also positively correlated with APTR LncRNA level (patients from our hospital: r |
| Databáze: | Complementary Index |
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