| Abstrakt: |
Major cell wall polysaccharides of the yeast cells comprises of mannan, p-glucan, and chitin. Each of these polysaccharide exhibits a variety of biological activities and can serve as medicine and functional foods. The methods for polysaccharide preparations are developing progressively. Previously, we had applied the heat degradation method at 135°C on yeast pglucan, which successively solubilized within several hours. In the present study, we applied the same method for intact Candida yeast cells. To compare the effects of the culture conditions, C-limiting (C-limit) and yeast extract-peptonedextrose medium (YPD) were used for cell culturing. After defatting by acetone and ethanol, the Candida cells (harvested from the two culture media) were suspended in distilled water separately and were autoclaved at 128°C. Following centrifiigation, the supernatants (C-CAWHE 1, Y - C A H W E 1) were dried. The Residues were suspended in distilled water and heat treated at 135°C. Further, the supernatants obtained after centrifiigation were dried (C-CAHWE 2，Y-CAHWE 2). The resulting residues were oxidized with sodium hypochlorite (NaCIO) and were subjected to a temperature of 135°C. The supernatants (C-CACLE, Y-CACLE) when dried, were analyzed by the chemical, biochemical, immunochemical, and biological methods. It was observed that 1) the heat treatment (135°C) was effective in extracting the mannan fraction, 2) p-glucans were effectively solubilized by combining the NaCIO and 135°C treatments, and 3) the cell wall architecture was brittle in C-limit cultured cells. The findings suggest the usefulness of the heat degradation method in preparing yeast polysaccharide(s), especially when used in parallel with NaCIO. [ABSTRACT FROM AUTHOR] |
