| Autor: |
Pierik, Antonio J., Duyvis, Martina G., van Helvoort, Joop M. L. M., Wolbert, Ronnie B. G., Hagen, Wilfred R. |
| Předmět: |
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| Zdroj: |
European Journal of Biochemistry; 4/1/92, Vol. 205 Issue 1, p111-115, 5p |
| Abstrakt: |
In addition to the 50-kDa (α) and 40-kDa (β) subunits, an 11-kDa polypeptide has been discovered in highly purified Desulfovibrio vulgaris (Hildenborough) dissimilatory sulfite reductase. This is in contrast with the hitherto generally accepted α2β2 tetrameric subunit composition. Purification, high-ionic-strength gel-filtration, native electrophoresis and isoelectric focussing do not result in dissociation of the 11-kDa polypeptide from the complex. Densitometric scanning of SDS gels and denaturing gel-filtration indicate a stoichiometric occurrence. A similar 11-kDa polypeptide is present in the desulfoviridin of D. vulgaris oxamicus (Monticello), D. gigas and D. desulfuricans ATCC 27774. We attribute an α2β2γ2 subunit structure to desulfoviridin-type sulfite reductases. N-terminal sequences of the α, β and γ subunits are reported. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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