| Autor: |
D'Arcy, Cleora J., Hewings, Adrianna D. |
| Předmět: |
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| Zdroj: |
Plant Pathology; Sep86, Vol. 35 Issue 3, p288-293, 6p |
| Abstrakt: |
Enzyme-linked immunosorbent assay (ELISA) systems were used to examine serological relationships and to detect three luteoviruses: a vector-non-specific strain of barley yellow dwarf virus from Illinois (BYDV-PAV-IL), a strain of beet western yellows virus from California (BWYV-CA) and a dwarfing strain of soybean dwarf virus from Japan (SDV-D). Indirect ELISA (IND-ELISA) systems detected distant reciprocal serological relationships among all three viruses. This is the first report of a serological relationship between a vector-non-specific strain of BYDV and any strain of SDV. Double antibody sandwich ELISA, (DAS-ELISA) systems detected the purified homologous viruses at concentration as low as 1.6 ng/ml, but did not detect heterologous viruses as concentrated as 800 ng/ml. In contrast, when DAS-ELISA systems were used for detection of the three viruses in sap extracts from infected plants some weak but significant (P=0.05) heterologous reactions occurred. The BYDV-PAV-IL-DAS-ELISA system usually detected BWYV-CA and sometimes detected SDV-D; the BWYV-CA DAS-ELISA system never detected BYDV-PAV-IL and rarely detected SDV-D; the SDV-D DAS-ELISA system sometimes detected BYDV-PAV-IL and consistently detected BWYV-CA. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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