| Abstrakt: |
Despite the progress in the search for causes of recurrent miscarrges, including the causes of Anti phospholipid syndrome, there is still an increasing need to improve diagnostic accuracy, so this study aims to determining allele frequency and gene polymorphism of the SNP 247 V/L of β2GPI patients with antiphospholipid syndrome. The study was carried out during the period expanded from December 2018 until the end of September 2018. About 80 women were included and divided according to their condition (APS influence) to: Patient (50 women who have undergone recurrent miscarriages) and 30 healthy women who have completed successful birth as a control group. Identification and genotyping of β2GPI gene by Real-Time Polymerase Chain Reaction (qRT-PCR) (Taqman-WizPureqRT-PCR Master SNP247 V/L of β2GPI genotyping assay). Two alleles (V and L) and three genotypes (VV, VL and LL) were identified in the examined subjects who. The allelic frequencies of b2GPI gene (V= 61%, L= 39%) as compared with control group, which were 13.4%, 13.4% respectively, with significant increase of L allele (P- value 0.001) and significant decrease in V allele (P- value 0.001) and p<0.01.The result of β2GPI gene polymorphism (SNP 247 V/L) statistically showed significant differences between APS patients and the control with significant increase in both LL and VL in patients with P- value 0.024, o.oo1 respectively and p<0.05, 0.01 respectively; while there were significant decrease in VV genotype in patient group with P-value 0.001 and p<0.01. In conclusion, SNP 247 V/L of b2GPI gene had a strong relationship in patients of APS (recurrent miscarriage women), with significant increase in T allele and significant decrease in G allele as compared with the control, with p<0.01. [ABSTRACT FROM AUTHOR] |
