| Autor: |
Chandel, Rahul Kumar, Sharma, Manju, Subramanian, S., Srivastava, Chitra, Khurana, S. M. Paul |
| Předmět: |
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| Zdroj: |
Biochemical & Cellular Archives; Apr2019, Vol. 19 Issue 1, p519-530, 12p |
| Abstrakt: |
The Lesser grain borer Rhyzopertha dominica (Fabricius) is one of the major coleopterous pests of stored grain products. Both larvae and adults are internal feeders, causing serious damages to grains, such as rice, maize, wheat and other stored commodities and causes serious losses to stored grains in tropical and subtropical region. Fumigation with phosphine gas is the main control method for R. dominica, but the indiscriminate and continuous use of phosphine gas has led to the development of resistance. Developing and implementing an effective phosphine resistance management strategy for R. dominica relies on an understanding of its genetic diversity and the structuring of that diversity geographically. Control over the infestation can be achieved through a better understanding of genetic diversity. Genetic diversity analyses are important because they can help us better understand evolutionary relationships that are relevant to agriculture, environment and human health. The mitochondrial cytochrome c oxidase I (mtCOI) exhibits reliable inter-species variations as compared to other markers. A total of fifteen population of R. dominica collected from different states of northern India, South Korea and Germany were investigated based on cytochrome c oxidase I (COI) mitochondrial DNA sequences. The results indicated that there are minor genetic variations among the populations at 520th and 531th positions. The populations from South Korea, Germany and Bareilly (BE-UP-01- IND) were found to be 100% genetically similar based on MtCOI gene sequences. Nucleotide analysis of genetic diversity and phylogenetic analysis of the COI gene sequences indicate that R. dominica from different geographical regions are homogenous. The results showed very less nucleotide diversity, number of haplotypes and haplotype gene diversity. Topologies of neighbourjoining (NJ) trees indicate all populations belong to single major clade. The result of this investigation suggests that the use of COI gene as a marker can provide a better estimate of genetic diversity and can be helpful to understand evolutionary processes and phosphine resistance mechanism. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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