| Abstrakt: |
Pseudomonas aeruginosa is an aerobic Gram-negative bacterium, which has feature as one of the utmostintricate nosocomial pathogens. that plays outstandingrole in infections wound of burned patients. Pseudomonas aeruginosa is an opportunistic pathogen whichas yetimprovelife-menacing infection in patients having fault in immunological system, such as patients which are burnt, particularly in developing countries. Even after this bacterium is resistant to numerous drugs surely and canobtain resistance to all antibiotics which are active, for this organism the contagionis generally problematic case for the patients. for the evaluation of susceptibility of drug and for multidrug resistant isolates determination this study was designed, and all isolates were analyzed for investigate plasmid profile.apply RAPD - PCR for finger print typing to determine the genotype variation. To characterizes P. aeruginosa strains that are widespread in patients in Iraq, Fivety two clinical samples were Isolates from Burns infected patient taken from Alyarmook and Ibn Albalady hospitals, by using culture methods, biochemical tests and confirmed by Apisystem API 20 NE ,were subjected to antibiotic susceptibilitypattren testing by determined using standard disc diffusion method, were shown high resistance for cefotaxime and low resistance forimipenem. All isolates were analyzed for investigate plasmid profile were detected in isolates The RAPD-PCR using the primer OPA-02. Then detection level CD4 and CD8 in serum burn paitients results showed CD4 and CD8 Concentration ng/ml were (167.044, 44.5522) mean (1.14± 0.016 P = 0.013, 1.0479± 0.02918 P = 0.001), respectively concluded identification of strain Pseudomonas aeruginosaat infection of burns can be done with use of RAPDand bacteria can be inducecellular immune response. [ABSTRACT FROM AUTHOR] |
