| Autor: |
Abdulhadi, Mohammed D., Obaid, Ayad A., Ibrahim, Kadhim M. |
| Předmět: |
|
| Zdroj: |
Biochemical & Cellular Archives; Apr2019, Vol. 19 Issue 1, p151-156, 6p |
| Abstrakt: |
An experiment was conducted with the aim of inducing genetic variation using the mutagen Ethyl Methane Solphonate (EMS) in Chrysanthemum morifoliumsingle nodes grown in vitro. Six concentrations of the mutagen were used (0, 0.5, 1.0, 1.5, 2.0, 2.5 or 3%). Two methods were implemented, either the EMS was dissolved in distilled water or in a buffer solution. Single nodes were soaked for 90 min with continuous shaking in all treatments except control. Results revealed a significant reduction in survival percentage correlated with the lowering of the solution pH. The LD50for the effect of the mutagen was determined in both methods recording 1% of the EMS in nodes soaked in distilled water whereas recorded 1.25% for those soaked in buffersolution. The experiment also investigated the genetic distance in number of mutated samples compared with mother plants using DNA markers with the aid of RAPD-PCR. DNA was extracted, isolated then purified before electrophoresis and implication. Results exhibited that EMS induced genetic variability particularly when single nodes were soaked in buffer solution containing the mutagen. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
