| Autor: |
Helynck, Gérard, Luu, Bang, Nussbaum, Jean-Louis, Picken, Douglas, Skalidis, George, Trifilieff, Elisabeth, Dorsselaer, Alain Van, Seta, Patrick, Sandeaux, Roger, Gavach, Claude, Heitz, Frédéric, Simon, Daniel, Spach, Gérard |
| Předmět: |
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| Zdroj: |
European Journal of Biochemistry; 7/1/83, Vol. 133 Issue 3, p689-695, 7p |
| Abstrakt: |
Proteolipid apoproteins have been isolated from a whole bovine brain homogenate by chloroform/methanol extraction, and fractionated by chromatography on modified (lipophilic) Sephadex, followed by ion-exchange chromatography on CM-Trisacryl. The various final, highly hydrophobic, fractions are homogeneous (sodium dodecyl sulfate/polyacrylamide gel electrophoresis). Transmembrane ion transfers were studied by 22Na+ flux and electrical conductance measurements. Single channel events were observed at low protein concentrations, in particular with one of the final homogeneous apoproteolipids of molecular mass 24 kDa. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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