| Autor: |
Sherrill, Hanna E., Jean, Philippe, Driver, Elizabeth C., Sanders, Tessa R., Fitzgerald, Tracy S., Moser, Tobias, Kelley, Matthew W. |
| Předmět: |
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| Zdroj: |
Journal of Neuroscience; 7/3/2019, Vol. 39 Issue 27, p5284-5298, 15p |
| Abstrakt: |
Acoustic signals are relayed from the ear to the brain via spiral ganglion neurons (SGNs) that receive auditory information from the cochlear inner hair cells (IHCs) and transmit that information to the cochlear nucleus of the brainstem. Physiologically distinct classes of SGNs have been characterized by their spontaneous firing rate and responses to sound and those physiological distinctions are thought to correspond to stereotyped synaptic positions on the IHC. More recently, single-cell profiling has identified multiple groups of SGNs based on transcriptional profiling; however, correlations between any of these groups and distinct neuronal physiology have not been determined. In this study, we show that expression of the POU (Pit-Oct-Unc) transcription factor Pou4fl in type I SGNs in mice of both sexes correlates with a synaptic location on the modiolar side of IHCs. Conditional deletion of Pou4fl in SGNs beginning in mice at embryonic day 13 rescues the early path-finding and apoptotic phenotypes reported for germline deletion of Pou4fl, resulting in a phenotypically normal development of SGN patterning. However, conditional deletion of Pou4fl in SGNs alters the activation of Ca2+ channels in IHCs primarily by increasing their voltage sensitivity. Moreover, the modiolar to pillar gradient of active zone Ca2+ influx strength is eliminated. These results demonstrate that a subset of modiolar-targeted SGNs retain expression of Pou4fl beyond the onset of hearing and suggest that this transcription factor plays an instructive role in presynaptic Ca2+ signaling in IHCs. [ABSTRACT FROM AUTHOR] |
| Databáze: |
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