| Autor: |
Kanegasaki, Shiro, Kojima, Yasuhiko, Matsuura, Motohiro, Homma, J. Yuzuru, Yamamoto, Akihiro, Kumazawa, Yoshio, Tanamoto, Ken-ichi, Yasuda, Tatsuji, Tsumita, Toru, Imoto, Masahiro, Yoshimura, Hiroyuki, Yamamoto, Michiharu, Shimamoto, Tetsuo, Kusumoto, Shoichi, Shiba, Tesuo |
| Předmět: |
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| Zdroj: |
European Journal of Biochemistry; 9/3/84, Vol. 143 Issue 2, p237-242, 6p |
| Abstrakt: |
Lipid A analogues were chemically synthesized based on the model structure recently revised, and biological activities of the analogues were tested. The analogue, (β-1,6)-linked glucosamine disaccharide carrying ester-bound 3-hydroxytetradecanoic acids at 3 and 3' position of reducing and nonreducing glucosamine in addition to amide-bound 3'-hydroxytetradecanoic acids and glycosidic-linked and ester-linked phosphate groups, showed much stronger activities for mediator inducing and immunomodulating as well as endotoxic activities than those exhibited by the previously synthesized analogues based on the old model. Among the activities tested, induction of interferon and tumor necrosis factor as well as mitogenicity, adjuvanticity and pyrogenicity were, however, not expressed so strongly as natural lipid A used as controls. In contrast, the analogue exhibited comparable activities to those of control lipid A in the test of lethal toxicity to mice and gelating activity of Limulus amebocyte lysate. Other synthetic analogues carrying a phosphate group showed comparable, slightly stronger or weaker activities depending on the test, but nonphosphorylated analogue exhibited no apparent or only very weak activities. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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