| Autor: |
Nuraini, Fatia Rizki, Setyaningsih, Ratna, Susilowati, Ari |
| Předmět: |
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| Zdroj: |
AIP Conference Proceedings; 2019, Vol. 2120 Issue 1, p080013-1-080013-9, 9p |
| Abstrakt: |
Mangifera casturi Kosterm is an endemic plant from South Kalimantan that has been listed as extinct in the wild by IUCN. Local society traditionally uses this plant to improve their overall health. In order to reduce further exploitation, the utilization of its endophytes is proposed as an alternative source of bioactive compounds for its medicinal worth. We investigated the antioxidant activity of the bioactive compounds of endophytic fungi isolated from M. casturi. Bioactive compounds of 13 endophytic fungal isolates were extracted using ethyl acetate and their antioxidant activity was evaluated using DPPH radical scavenging method. TLC-bioautography assay and GC-MS analysis were used to detect the antioxidant compounds present in the extracts. The endophytic fungi were identified morphologically and molecularly based on ITS rDNA sequence. Among 13 isolates, Aspergillus minisclerotigens AKF1 and Aspergillus oryzae DK7 exhibited the strongest antioxidant capacity with an IC50 value of 142.96μg/mL and 145.01μg/mL, respectively. Based on TLC-bioautography and GC-MS analysis, the most active compound in AKF1 and DK7 isolate was dihydropyran and 4H-Pyran-4-one,5-hydroxy-2-(hydroxymethyl-(CAS) Kojic acid, respectively. This finding confirms the potential of endophytic fungi as a source of antioxidants and can be an alternative to the antioxidants produced by plants to support M. casturi conservation. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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