| Abstrakt: |
During the interaction of bacteria or other particles with neutrophils, oxygen consumption is increased, and unstable reduction products are produced. Chemiluminescence is the light energy produced in the neutrophil by the generation of unstable oxygen radicals. These oxygen radicals are thought to be important in the destruction of bacteria as well as host tissues. Therefore, we wanted to investigate the ability of Gram-negative plaque bacteria to stimulate neutrophil chemiluminescence in vitro. The major groups of bacteria examined were Fusobacterium, Bacteroides, Capnocytophaga, Selenomonas, and Treponema. Of these bacteria, Fusobacterium nucleatum had by far the greatest ability to stimulate neutrophil chemiluminescence in the absence of serum. Our results suggest that stimulation of neutrophil chemiluminescence by F. nucleatum is mediated by a protein moiety on the bacterial cell surface. This conclusion is supported by experiments demonstrating significant inhibition of neutrophil chemiluminescence by heat, 2% formalin, or trypsin pre-treatment of F. nucleatum. Also, neutrophil chemiluminescence was stimulated in a similar fashion with intact F. nucleatum only by the cell-association centrifugation pellet of sonicated F. nucleatum. The monosaccharide D-galactose, which is a component of erythrocyte glycoproteins and F. nucleatum lipopolysaccharide, caused significant inhibition of neutrophil chemiluminescence at 100 mM final concentration. The unusual and pronounced ability of Fusobacterium nucleatum to stimulate neutrophil chemiluminescence in the absence of serum might be important in the pathogenicity and/or host defense in periodontal disease. [ABSTRACT FROM AUTHOR] |
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