| Autor: |
de Swart, Rik L., de Vries, Rory D., Rennick, Linda J., van Amerongen, Geert, McQuaid, Stephen, Verburgh, R. Joyce, Yüksel, Selma, de Jong, Alwin, Lemon, Ken, Nguyen, D. Tien, Ludlow, Martin, Osterhaus, Albert D. M. E., Duprex, W. Paul |
| Předmět: |
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| Zdroj: |
NPJ Vaccines; 2017, Vol. 2 Issue 1, pN.PAG-N.PAG, 1p |
| Abstrakt: |
Needle-free measles virus vaccination by aerosol inhalation has many potential benefits. The current standard route of vaccination is subcutaneous injection, whereas measles virus is an airborne pathogen. However, the target cells that support replication of live-attenuated measles virus vaccines in the respiratory tract are largely unknown. The aims of this study were to assess the in vivo tropism of live-attenuated measles virus and determine whether respiratory measles virus vaccination should target the upper or lower respiratory tract. Four groups of twelve cynomolgus macaques were immunized with 104 TCID50 of recombinant measles virus vaccine strain Edmonston-Zagreb expressing enhanced green fluorescent protein. The vaccine virus was grown in MRC-5 cells and formulated with identical stabilizers and excipients as used in the commercial MVEZ vaccine produced by the Serum Institute of India. Animals were immunized by hypodermic injection, intra-tracheal inoculation, intra-nasal instillation, or aerosol inhalation. In each group six animals were euthanized at early time points post-vaccination, whereas the other six were followed for 14 months to assess immunogenicity and protection from challenge infection with wild-type measles virus. At early time-points, enhanced green fluorescent protein-positive measles virus-infected cells were detected locally in the muscle, nasal tissues, lungs, and draining lymph nodes. Systemic vaccine virus replication and viremia were virtually absent. Infected macrophages, dendritic cells and tissue-resident lymphocytes predominated. Exclusive delivery of vaccine virus to the lower respiratory tract resulted in highest immunogenicity and protection. This study sheds light on the tropism of a live-attenuated measles virus vaccine and identifies the alveolar spaces as the optimal site for respiratory delivery of measles virus vaccine. Measles: The efficacy of needle-free vaccine delivery Research shows that measles aerosol vaccination is effective when the vaccine is delivered to the lower respiratory tract. Measles virus is a highly contagious and potentially deadly human pathogen, but is easily prevented by vaccination. An international team led by Paul Duprex, of the United States' Boston University School of Medicine, and Rik de Swart, of the Erasmus University Medical Center, The Netherlands, investigated the effectiveness of different routes of measles vaccine administration in non-human primates. They found that macaques responded well to aerosolized administration of the live-attenuated measles vaccine and they were effectively protected from subsequent measles virus infection. Vaccine delivery to the lower respiratory tract was most effective in eliciting protective immune responses. This research is a step towards easier vaccination protocols and away from dependency on sterile needles and trained health-care workers — an important consideration in the developing world. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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