| Autor: |
van Beelen, Patrick, Labro, Joannes F. A., Keltjens, Jan T., Geerts, Wim J., Vogels, Godfried D., Laarhoven, Wim H., Guijt, Wim, Haasnoot, Cornelis A. G. |
| Předmět: |
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| Zdroj: |
European Journal of Biochemistry; 3/1/84, Vol. 139 Issue 2, p359-365, 7p |
| Abstrakt: |
Degradational studies of methanopterin, a coenzyme involved in methanogenesis, are reported. The results of these studies are in full accordance with the proposed structure of methanopterin as N-[l′-(2″-amino-4″-hydroxy- 7″-methyl-6″-pteridinyl)ethyl]-4-[2′,3′,4′,5′--tetrahydroxypent-1′-yl(5′1″)O-α-ribofuranosyl-5″-phosphoric acid] aniline in which the phosphate group is esterified with α-hydroxyglutaric acid. Acid hydrolysis of methanopterin cleaved the 5′ → 1″ glycosidic bond and yielded a ‘hydrolytic product’ which was identified as N-[1′-(2″-amino-4″- hydroxy-7″-methyl-6″-pteridinyl)ethyl]-4-[2′,3′,4′,5′-tetrahydroxypent-1′-yl]aniline. Alkaline permanganate oxidation of methanopterin yielded 7-methylpterin-6-carboxylic acid. Catalytic (or enzymatic) hydrogenation of methanopterin gave a mixture of 6-ethyl-7-methyl-7.8-dihydropterin. 6-ethyl-7-methylpterin and a third com- pound, named methaniline which was identified as 4-[2′,3′,4′,5′-tetrahydroxypent-1′-yl(5′ → 1″)O-α-ribofuranosyl- 5″-phosphoric acid]aniline, in which the phosphate group is esterified with α-hydroxyglutaric acid. Methanosarcina barkeri contains a closely related coenzyme called sarcinapterin, which was identified as a L-glutamyl derivative of methanopterin, where the glutamate moiety is attached to the α-carboxylic acid group of the α-hydroxyglutaric acid moiety of methanopterin via an amide linkage. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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