| Autor: |
Gaudenzi, Silvia, Pozzi, Deleana, Toro, Paolo, Silvestri, Ida, Morrone, Stefania, Castellano, Agostina Congiu |
| Předmět: |
|
| Zdroj: |
Spectroscopy: An International Journal; 2004, Vol. 18 Issue 3, p415-422, 8p |
| Abstrakt: |
We used Fourier Transform Infrared Spectroscopy (FTIR) combined with flow cytometry to study the apoptosis and necrosis processes in Jurkat, a lymphocyte cell line. The apoptosis was induced in the cells by a chemical agent, the actinomycin D, while the necrosis was induced lowering the pH value to 4.2. The apoptotic events were analysed by flow cytometry (using annexin V and propidium iodide) and contemporary monitored by FTIR spectroscopy at different times after the treatment. This comparison allowed us to find in the IR spectrum, between 3000 cm-1 and 2800 cm-1, a “marker band” of the apoptosis corresponding to the exposure of phosphatidylserine on the outer leaflet of the membrane. A marker of a specific cellular process obtained by using a non‐destructive technique such as FTIR spectroscopy, has a great significance in the diagnostic medicine providing a tool for detecting pathologies in vivo. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
