In vivo evaluation of the genotoxicity and oxidative damage in individuals exposed to 10% hydrogen peroxide whitening strips.

Autor: Del Real García, José Francisco, Saldaña-Velasco, Fausto Rubén, Sánchez-de la Rosa, Susana Vanessa, Ortiz-García, Yveth Marlene, Morales-Velazquez, Gabriela, Gómez-Meda, Belinda Claudia, Zúñiga-González, Guillermo Moisés, Sánchez-Parada, María Guadalupe, Zamora-Perez, Ana Lourdes
Zdroj: Clinical Oral Investigations; Jul2019, Vol. 23 Issue 7, p3033-3046, 14p, 1 Color Photograph, 1 Diagram, 1 Chart, 7 Graphs
Abstrakt: Objective: This study assessed the impact of 10% hydrogen peroxide whitening strip exposure on the genotoxicity and oxidative damage by means of the buccal micronucleus cytome assay by counting nuclear abnormalities (NAs) in buccal mucosa and attached gingiva cells and by analyzing in whole saliva the molecule 8-hydroxy-2′-deoxyguanosine (8-OHdG). Materials and methods: The study was conducted on 113 subjects divided into two groups: group 1 or control (n = 53), non-whitening strip exposed, and group 2 (n = 60), whitening strip exposed (Crest® 3D Whitestrips® premium plus, 10% hydrogen peroxide). Oral epithelial cells and whole saliva samples were taken at the beginning and 30 days later for group 1 and immediately before bleaching and 15 and 30 days after the end of the bleaching for group 2. Results: An increased frequency of NAs (p < 0.05) and higher levels of 8-OHdG (p < 0.05) were observed after bleaching exposure. Also, a positive correlation exists between oxidative stress produced by hydrogen peroxide and micronuclei was found. Conclusion: Individuals exposed to 10% hydrogen peroxide whitening strips exhibit NAs increased in oral epithelial cells and 8-OHdG in saliva, which is directed related to nuclear and oxidative DNA damage, respectively. Clinical significance: Hydrogen peroxide is the active agent of tooth whitening and this compound induced DNA damage. Individuals exposed to whitening strips with 10% hydrogen peroxide exhibit increased genotoxic and oxidative damage. Therefore, self-application of bleaching agents should be handled carefully since it could be a risk to human health. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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