| Abstrakt: |
The goal was to prove by complex morphological analysis the purposefulness of using a biocomposition based on silver nanoparticles stabilized in solution with sodium alginate and the inhibitor of proteases of aprotinin for influencing the course of the inflammatory process after thermal burn. Using light and electron microscopy we studied morphological changes after thermal skin damage of II grade in rats using biocomposite based on silver nanoparticles stabilized in solution with sodium alginate and the protease inhibitor aprotinin to modify the course of the inflammatory process. Research was performed on 80 white male Wistar rats, 180-200g weight. The early wound process (3rd day) did not differ in various groups and was characterized by the predominance of exudative-necrotic and dyscirculatory disorders over the regeneration processes. From the 7th day, the morphological picture began to differ depending on the applied medication therapy. There was noted the beginning of rejection of the scab, a decrease in the intensity of inflammation, in hemodynamic disturbances and the onset of epithelialization. On the 14th day, the epithelialization was observed, the inflammatory process was decreasing and the young scar was forming. The use of erythromycin facilitated the migration of keratinocytes and accelerated the restoring of connective tissue in dermis through synthetic activity of fibroblasts. The use of the silver nanoparticle composition had a moderate corrective effect. After proteinase inhibitors application, the dystrophic changes, both in the epidermis and in dermis, persisted longer than in other groups. At the same time, samples where burns were treated by combined way (the solution of nano-silver and an inhibitor of proteases of aprotinin) demonstrated morphological signs of the increase in the keratinocytes proliferative activity and functional activation of protein synthesis of fibroblasts. According to the results of light and electron microscopy, the best result was obtained after combined therapy; the morphological characteristic of wounds was somewhat worse after antibiotic or silver monotherapy. The monotherapy only with the proteinase inhibitor gave the worst results on the duration of healing and the severity of inflammation in the wound. The local application of the silver nanoparticles stabilized in sodium alginate solution and aprotinin limited the destructive changes in the burnt skin, assisted in early repair processes, helped in the formation of granulation tissue, its maturation and epithelization. [ABSTRACT FROM AUTHOR] |
