| Abstrakt: |
The utilization and turnover of injected S35-sulfate by the connective tissue of the periodontium was studied in 28 IRC strain mice, 14 one month old and 14 one year old. The isotope was administered by intraperiotoneal injection in a dosage of 20 uc of sodium S35-sulfate per gram of body weight. The animals were sacrificed 1, 4 and 12 hours, 1 and 4 days and 1 and 2 months after the injection. Following fixation and decalcification, the tissues of the head were processed by histologic, radioautographic and enzymatic digestion techniques: Analysis of the results permitted the drawing of the following conclusions: 1) the injected S35-sulfate did label certain of the sulfated mucopolysaccharides of the amorphous ground substance of the periodontal ligament, cementum, alveolar bone and dentin: 2) the fibroblasts, cementblasts, osteoblasts and odontoblasts apparently take part in the synthesis of the sulfated mucopolysaccharides, 3) the labeled sulfated mucopolysaccharides in the periodontal ligament turned over rapidly and the rate did not appear to decrease with age, 4) within the mineralized matrix of the alveolar bone, dentin or cementum no turnover of the sulfated mucopolysaccharides was detected; and 5) cementoblastic, osteoblastic and odontoblastic synthetic activity in regards to the utilization of S35 sulfate decreased with age except in the case of the continually erupting incisior. [ABSTRACT FROM AUTHOR] |
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