| Autor: |
Levinson, G, Keyvanfar, K, Wu, J C, Fugger, E F, Fields, R A, Harton, G L, Palmer, F T, Sisson, M E, Starr, K M, Dennison-Lagos, L |
| Předmět: |
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| Zdroj: |
Human Reproduction; Apr1995, Vol. 10 Issue 4, p979-982, 4p |
| Abstrakt: |
We report the world's first clinical pregnancy resulting from DNA-based enrichment for X-bearing human spermatozoa, for prevention of X-linked hydrocephalus. Sperm separation was followed by embryo biopsy and nested multiplex polymerase chain reaction (PCR) for gender determination. Enriched populations of X-bearing spermatozoa ranging from 80 to 89% pure as determined by fluorescence in-situ hybridization (FISH) resulted in in-vitro fertilization (IVF) rates indistinguishable from normal IVF procedures (65%). In two separate biopsy procedures, 7/9 and 15/16 of the resulting embryos were determined to be female by multiplex PCR. Embryo transfer resulted in a karyotypically normal female fetus. This technique should be widely applicable to gender selection for the prevention of genetic disorders. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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