| Autor: |
Hawley, Charles E., Falkler Jr., William A. |
| Předmět: |
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| Zdroj: |
Journal of Periodontal Research; 1979, Vol. 14 Issue 5, p390-396, 7p |
| Abstrakt: |
A complement consumption assay was used to investigate the anti-complementary activity (AC) of Fusobacterium polymorphum (nucleatum) in guinea pig complement (GPC) which had been treated with the divalent cation chelators ethylamine diamine tetra acetic acid (EDTA) or ethylene glycol tetra acetic acid (EUTA). In the presence of 1mM EGTA, a cell wall preparation of F. polymorphuin reduced the hemolytic complement activity of GPC to essentially the same degree as that observed in the non-chelated serum controls. There was no classical pathway complement activity in the EGTA treated sera against hemolysin sensitized sheep erythrocytes (EA). A 1 mM concentration of EDTA inhibited both the AC activity of F. polymorphum cell walls and the lysis of EA via the classical pathway. It was also shown that the addition of 1 mM magnesium ion (Mg++) reversed the inhibitory effects of 1 mM EDTA on the lysis of EA and on the apparent alternative pathway activity displayed by F. polymorphum cell walls. In contrast, the same concentration of Mg++ in the 1mM EGTA tests did not reverse the EGTA inhibition of the classical pathway, nor did it markedly augment the alternative pathway. These results indicate that the observed AC activity demonstrated by the cell wall preparation of F. polymorphum did not depend upon a functional classical pathway, but that it probably occurred exclusively via the alternative complement pathway. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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