| Autor: |
Martin, Stephen A., Falkler, William A., Jr., Suzuki, Jon B., Hawley, Charles E., Mackler, Bruce F. |
| Předmět: |
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| Zdroj: |
Journal of Periodontal Research; Jul1986, Vol. 21 Issue 4, p351-364, 14p |
| Abstrakt: |
The purpose of this study was th compare the reactivity of the localized immunoglobulins present in the supernatant fluids from in vitro gingival organ cultures of rapidly progressive (RP) and adult periodontitis (AP) patients with those immunoglobulins occurring systemically in the sent of these patients. Tissue excised from periodontitis patients was diagnosed and classified as being either RP or AP and placed in the wells of a multiwell microtiter plate to which RPMI 1640 tissue culture medium was added. Supernatant fluids were harvested and fresh culture medium added to the wells at 24 h intervals. Supernatant fluids and autologous sera from RP and AP patients were tested for reactivity to Bacteroides gingivalis, an organism often implicated in periodontitis, as well as B. asaccharolyticus, a non-oral, non-crossreacting member of the genus Bacteroides, utilizing an ELISA technique. When immunoglobulins present in gingival tissue supernatant fluids were examined, it was found that mean levels reactive to B. gingivalis were significantly greater (p < 0.05) than those to B. asaccharolyticus regardless of patient group. Similarly, the AP group always exhibited significantly (p <0.05) increased antibody reactivity to B. gingivalis when compared to the RP periodontitis patients. However, when autologous sera from these patients were assayed, no significant differences were observed for immunoglobulin levels reactive to B. gingivalis or B. asaccharolyticus when comparing the AP and RP patient groups, or within the AP group alone. The results indicate that differences exist in the localized immunological responses of RP and AP patients to the periodontopathogen B. gingivalis that are not necessarily reflected in the systemic serum antibody responses of these patients. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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