| Autor: |
Bathurst, Ian C., Craig, Roger K., Herries, David G., Campbell, Peter N. |
| Předmět: |
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| Zdroj: |
European Journal of Biochemistry; 8/1/80, Vol. 109 Issue 1, p183-191, 9p |
| Abstrakt: |
RNA complexity analysis of poly(A)-containing RNA isolated from the lactating guinea-pig mammary gland demonstrates that the complexity within the nuclear population was three—four-fold greater than that in the equivalent post-nuclear polyribosomal population [see Craig et al., Biochem. J. (1979) 181, 737–756]. Up to 21000 different sequences distributed in two abundance groups were detected in the nucleus, whereas 5000 sequences distributed in three abundance groups were present in the post-nuclear population. All poly(A)-containing RNA sequences present in the post-nuclear fraction could be detected in the nuclear poly(A)-containing population. Analysis of the relative distribution of the three post-nuclear abundance populations within the nuclear poly(A)-containing RNA population demonstrates that the abundant and moderately abundant post-nuclear sequences were present at similar concentrations within the nucleus, and comprised the abundant nuclear population. The abundant and moderately abundant post-nuclear sequences were present at 62200 and 785 copies of each sequence/cell in the post-nuclear fraction respectively, and 44 and 118 copies of each sequence/cell in the nuclear fraction respectively. The scarce post-nuclear sequences (18.5 copies of each sequence/cell) were also present at low levels in the nuclear fraction (0.1 copy of each sequence/cell). The results are discussed in terms of the post-transcriptional regulation of gene expression in the lactating guinea-pig mammary gland. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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