| Abstrakt: |
Objective This study was conducted to investigate the effects of Artemisia extract on some immunological parameters in streptozotocininduced diabetic mice. Methods After preparation of Artemisia extract, many chemical tests were used to identify the type of element and compounds presented in this plant using many chemical techniques. Thirty five streptozocin (STZ)-induced diabetes mice were divided into five groups; the first group provided only with water, the other four groups were consumed orally ingested the plant extract in four different concentration (2000, 1000, 500, 250) mg/kg of body weight. Another 10 mice did not injected with STZ were divided into two groups; one consumed Artemisia (Art group), and the second consumed only normal saline (Cont. group). After 14 days of diabetes induction and Artemisia extract treatment, the mice were sacrificed. Blood and tissue (brain, spleen and kidney) were collected. Fasting blood sugar and insulin levels were determined in the serum. Furthermore tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) levels were determined in the serum and aliquots of homogenize tissues. Results Results declared that the extract of Artemisia fruit contains high levels of active compounds especially antioxidant compounds. IL-6 and TNF-α levels were decreased while insulin and glucose levels were increased in the STZ-induced mice group. Artemisia extract effects differently on glucose and insulin levels depending on its concentration. Interestingly, IL-6 and TNF-α levels increase in serum, brain, and spleen of the STZ-induced mice group consumed different concentration of Artemisia but it normalized in the STZinduced mice group consumed 250 mg/kg Artemisia, as well as insulin and glucose levels for the same group, while there was no difference in kidney. Conclusion Artemisia can control diabetes in 1000 and 500 mg/kg through controlling insulin level, and in the other hand, using the plant extract in 250 mg/kg, acts as an immune modulator for anti-inflammatory agents like IL-6 and TNF-α. [ABSTRACT FROM AUTHOR] |
