| Autor: |
Matthews, Harry R., Johnson, Edward M., Steer, Wendy M., Bradbury, F. Morton, Allfrey, Vincent G. |
| Předmět: |
|
| Zdroj: |
European Journal of Biochemistry; 1/16/78, Vol. 82 Issue 2, p569-576, 8p |
| Abstrakt: |
Netropsin binds to DNA in caesium chloride density gradients and reduces the density of the DNA. The DNA is saturated at a netropsin/DNA weight ratio of about 6 and the change in density, Δ..., at saturation is given by Δ... = - 109 (dA + dT content)1.87 mg/ml for the six DNAs tested covering dA +dT contents from 0.28 to 0.69. At lower netropsin/DNA ratios the observed density shifts are consistent with a two-site model for netropsin binding to DNA. Netropsin approximately doubles the resolution of Physarurn polycephalum nucleolar satellite DNA from main-band DNA. The fragments of P. polycephalum nucleolar satellite DNA obtained with the restriction endonuclease HindIII do not separate on CsCl gradients, even in the presence of netropsin, which shows that the transcribed and non-transcribed sequences in this DNA have similar nucleotide compositions. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
