| Autor: |
Tan, S.S., O'Toole, E.M., Kurtz, C.B., Weis, J.H. |
| Předmět: |
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| Zdroj: |
Immunology; May93, Vol. 79 Issue 1, p82-88, 7p |
| Abstrakt: |
The murine Cr2 gene encodes mRNA that produce two protein products predicted to be approximately 145,000 Mr (Cr2-145) and 190,000 Mr (Cr2-190). All cells examined which express the Cr2 gene produce transcripts encoding both the Cr2-145 and Cr2-190 proteins: both transcripts are constitutively expressed by mature B cells. To determine if Cr2 expression could be altered by activating splenic B cells, splenic cultures were incubated with lipopolysaccharide (LPS) and cell surface Ig chains were cross-linked with anti-μ. In the presence of LPS and anti-μ both Cr2 and Oct-2 transcripts were diminished while the control β-actin transcript levels remained unchanged. However, when LPS alone was added, only the Cr2 transcript levels were diminished. To test if these findings could be reproduced in vivo, animals were provided with a peritoneal injection of either Escherichia coli or Listeria monocytogenes and transcript levels analysed. The quantities of both Cr2 transcripts, as well as those encoding Oct-2, were substantially reduced in splenocytes and peripheral lymphatic tissues obtained from these infected mice while those encoding the mouse Crry protein, the B-cell marker CD19 and β-actin remained unchanged. These data suggest that when confronted with a major bacterial infection, murine B cells respond by shutting down synthesis of transcripts encoding the Cr2 and Oct-2 gene products. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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